Table 2: mRNA levels of selected genes involved in heart development in P19CL6 cell lines with and without induction to generate a cardiomyocyte phenotype1.

GeneCell line2mRNA level: noninduced cells (U)mRNA level: induced cells (I)I/U3

SRFMock clones #1, 2, 31.08 ± 0.110.46 ± 0.080.43 ± 0.01
mRhoAN19 clone #10.60 ± 0.121.68 ± 0.11*2.75 ± 0.06*
Cardiac -actin Mock clones #1, 2, 30.97 ± 0.040.63 ± 0.170.65 ± 0.02
mRhoAN19 clone #11.14 ± 0.142.93 ± 0.35*2.57 ± 0.04*
GATA4Mock clones #1, 2, 31.00 ± 0.480.35 ± 0.050.35 ± 0.04
mRhoAN19 clone #10.70 ± 0.122.41 ± 0.38*3.44 ± 0.09*

Data represent the results of 3–6 independent measurements.
Values for mock transfected (Mock) clones #1, 2, 3 represent combined results for these three cell lines.
I/U is the ratio of mRNA levels in induced (I) and uninduced (U) cells.
, compared to mock transfected cell lines.