Promising Noninvasive Cellular Phenotype in Prostate Cancer Cells Knockdown of Matrix Metalloproteinase 9
Figure 1
Determination of the expression of CD44s, v6, and v10 in PC3 cells knockdown of MMP9: PC3 cells transfected with vector DNA (PC3/V), scrambled nonsilencing SiRNA (PC3/Sc) and silencing SiRNA (PC3/Si) were used for real time PCR (a) and immunoblotting analyses (b–f). Untransfected PC3 cells were also used as controls. The expression levels of CD44s, CD44v6, and CD44v10 mRNA were determined by real time PCR analysis and normalized relative to GAPDH expression (a). **;* versus indicated respective controls (PC3/V and PC3/Sc). Equal amount of protein was used for immunoblotting analysis with an antibody to CD44v6 (b and e) and CD44v10 (c). A shorter exposure blot for PC3/Si cells is shown in lane 5. The blot in (b) was stripped once and probed successively with an antibody to CD44v10 (c) and GAPDH (d). The blot in (e) was stripped and reprobed with an antibody to GAPDH. The levels of GAPDH represent the loading control for each experiment set. The results shown are representative of three or four experiments.
