Association between TLR4 and TLR9 Gene Polymorphisms with Development of Pulmonary Tuberculosis in Zahedan, Southeastern Iran

Table 1

Primers, PCR conditions, and restriction enzymes used for genotyping TLRs genes.

SNPs product	Primer sequence 5′-3′	AT	PCR product	RE
TLR4
Arg299Gly	F: GATTAGCATACTTAGACTACTACCTCCATG	60°	249 bp	NcoI
	R: GATCAACTTCTGAAAAAGCATTCCCAC
Thr399Ile	F: GGTTGCTGTTCTCAAAGTGATTTTGGGAGAA	61°	406 bp	HinfI
	R: CCTGAAGACTGGAGAGTGAGTTAAATGCT
TLR9
T-1486C	F: TTCATTCAGCCTTCACTCAG	55°	558 bp	BslI
	R: TCAAAGCCACAGTCCACAG

F: forward primer; R: reverse primer; AT: annealing temperature; RE: restriction enzyme.