Research Article
Application of HPLC with ELSD Detection for the Assessment of Azelaic Acid Impurities in Liposomal Formulation
Table 1
Results of the assessment of the precision of the chromatographic system due to the assessments of nine identified impurities of the azelaic acid.
| Measurement | Peak area (A) for the assessed identified impurities: | I1 | I2 | I3 | I4 | I5 | I6 | I7 | I8 | I9 |
| 1 | 258.113 | 1203.014 | 1706.031 | 1746.713 | 2830.966 | 2752.13 | 4702.942 | 3528.136 | 3821.195 | 2 | 267.883 | 1201.028 | 1717.689 | 1736.138 | 2809.523 | 2760.488 | 4735.845 | 3499.348 | 3827.302 | 3 | 269.431 | 1207.302 | 1710.66 | 1735.395 | 2805.159 | 2751.771 | 4728.282 | 3506.829 | 3831.149 | 4 | 261.999 | 1208.041 | 1717.319 | 1757.147 | 2842.288 | 2740.871 | 4701.934 | 3511.741 | 3804.322 | 5 | 265.122 | 1193.801 | 1715.516 | 1746.554 | 2801.162 | 2787.357 | 4698.756 | 3501.03 | 3805.952 | 6 | 259.204 | 1204.02 | 1698.914 | 1756.218 | 2805.766 | 2754.597 | 4730.213 | 3490.329 | 3821.493 | 7 | 266.327 | 1193.193 | 1696.215 | 1744.038 | 2824.143 | 2751.029 | 4736.137 | 3493.413 | 3844.485 | 8 | 258.348 | 1201.998 | 1699.815 | 1743.793 | 2829.951 | 2765.384 | 4703.253 | 3532.354 | 3824.421 |
| | 263.303 | 1201.550 | 1707.770 | 1745.750 | 2818.620 | 2757.953 | 4717.170 | 3507.898 | 3822.540 | SD | 4.49 | 5.53 | 8.75 | 7.99 | 15.14 | 13.88 | 16.77 | 15.41 | 13.05 | RSD | 1.7 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.4 | 0.4 | 0.3 |
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I1–I9: identified impurities, : average, SD: standard deviation, SRD: relative standard deviation.
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