Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase

Figure 1

Effect of various polyols, such as, glucose (GLC), trehalose (TRE), glycerol (GLY), and ethylene glycol (EG), on the far-UV CD spectra of the native and the acid-denatured GAs. Different line symbols represent native GA (thick curve), acid-denatured GA (thin curve), native GA + polyol (dashed curve), and acid-denatured GA + polyol (dotted curve). The spectra were recorded at 25°C using a protein concentration of 1.4 μM. Different polyol concentrations used were 2.6 M GLC, 1.3 M TRE, 8.0 M GLY, and 8.0 M EG.