Research Article

Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase

Figure 5

Far-UV CD spectra of the native (thick curve) and the thermal-denatured GAs in the absence (thin curve) and the presence of 2.6 M GLC (dashed curve), 1.3 M TRE (dotted curve), 8 M GLY (dashed with one dot curve), and 8 M EG (dashed with two dots curve). The spectra of the thermal-denatured GA were recorded after equilibrating the sample at 71°C for 6 min, using a protein concentration of 1.4 μM.
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