Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase

Table 2

Effect of various polyols on the fluorescence spectral characteristics of different states of GA.

Polyols	N state; (pH 7.0, 25^∘C)			AD state  (pH 1.0, 25^∘C)
	^1𝐸𝑚  (nm)	^2FI340nm	^3ΔFI (%)	𝐸𝑚  (nm)	FI342nm	ΔFI (%)
—	340	222	—	342	143	—
2.6 M GLC	338	195	−12.2	341	214	49.7
1.3 M TRE	338	201	−9.5	340	208	45.5
8.0 M GLY	339	218	−1.8	343	247	72.7
8.0 M EG	339	256	+15.3	344	277	93.7

¹𝐸_𝑚: Emission maxima.
²FI: Fluorescence intensity at 340/342 nm.
³ΔFI: Percentage change in the fluorescence intensity in the presence of polyols.