Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase

Table 3

Thermodynamic parameters for thermal denaturation of glucoamylase as monitored by CD spectroscopy.

Glucoamylase	𝑇𝑚  (^∘C)	ΔH; (KJ mol^−1)	ΔG  (25^∘C); (KJ mol^−1)	ΔΔ𝐺^a  (%)
Native (N)	64.3	264.30	30.79	—
N + 2.6 M GLC	77.2	365.56	55.52	80.3
N + 1.3 M TRE	75.3	326.77	48.12	56.3
N + 8.0 M GLY	66.9	283.72	36.74	19.3
N + 8.0 M EG	53.2	307.02	26.48	−14.0

aΔΔ𝐺 represents percentage change in the Δ𝐺 value in the presence of polyols. Negative sign shows the decrease.