Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase

Table 4

Effect of various polyols on the CD and fluorescence spectral characteristics of the thermal-denatured GA at 71^∘C.

Polyols	CD		Fluorescence
	MRE222nm	^1ΔMRE222nm  (%)	^2𝐸𝑚 (nm)	^3FI340nm	^4ΔFI (%)
—	−5420	—	349	103	—
2.6 M GLC	−11404	110.4	340	111	7.8
1.3 M TRE	−10360	91.1	341	113	9.7
8.0 M GLY	−6624	22.2	344	130	26.2
8.0 M EG	−5104	−5.8	352	149	44.7

1ΔMRE_222nm represents percentage change in the MRE_222nm in the presence of polyols. Negative sign shows the decrease.
²𝐸_𝑚 = Emission maxima.
³FI_340nm = Fluorescence intensity at 340 nm.
⁴ΔFI = Percentage change in the fluorescence intensity in the presence of polyols.