Research Article

Fungal and Bacterial Communities in the Rhizosphere of Pinus tabulaeformis Related to the Restoration of Plantations and Natural Secondary Forests in the Loess Plateau, Northwest China

Table 1

Primers used for nested PCR amplification of soil fungal and bacterial communities in the rhizosphere of Pinus tabulaeformis.

Primer namePrimer sequence (5′-3′)Reference

Fungi
 ITS1-FCTTGGTCATTTAGAGGAAGTAAGardes and Bruns [25]
 ITS4TCCTCCGCTTATTGATATGCWhite et al. [26]
 ClampCGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGGGardes and Bruns [25]
 ITS1-F-GCClamp-CTTGGTCATTTAGAGGAAGTAAGardes and Bruns [25]
 ITS2GCTGCGTTCTTCATCGATGCWhite et al. [26]
Bacteria
 fD1AGAGTTTGATCCTGGCTCAGWeisburg et al. [27]
 rP1ACGGTTACCTTGTTACGACTTWeisburg et al. [27]
 341fCCTACGGGAGGCAGCAGMuyzer et al. [28]
 341f-GCClamp-CCTACGGGAGGCAGCAGMuyzer et al. [28]
 534rATTACCGCGGCTGCTGGMuyzer et al. [28]