Relevance of Biofilms in the Pathogenesis of Shiga-Toxin-Producing Escherichia coli Infection

Figure 1

Quantification of biofilm formation of STEC strains by crystal violet (CV) staining expressed in biofilm biomass units (BBU): (a) in TSB; with addition of 0.5% glucose; in thioglycollate medium alone and with the addition of 0.5% glucose. (b) ROS ratio (ROS/BBU) determined by NBT assay. (c) NO and BBU ratio (NO/BBU) determined by Griess method. Error bars represent the standard deviations of the means of three independent experiments. ^#𝑃 versus TSB<0.01 and ^#𝑃 versus thioglycollate medium<0.01 being considered statistically significant.

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