Insights on the Phytochemical Profile (Cyclopeptides) and Biological Activities of Calotropis procera Latex Organic Fractions

Table 2

Evaluation of toxicity potential of fractions of C. procera latex against cell lines and Brine shrimp nauplii.

Fractions	Sample (LC50  μg·mL^−1)
	MTT assay				Brine shrimp assay
	HL-60	Ovcar-8	HCT-116	SF-295
Hexane	2.9	6.5	3.8	3.2	781.5±31.2
	(2.4–3.4)	(5.6–7.6)	(3.5–4.1)	(2.7–3.8)	(721.2–843.3)
Dichloromethane	0.05	0.17	0.11	0.12	10.9±0.9
	(0.04–0.06)	(0.14–0.2)	(0.1–0.13)	(0.1–0.14)	(9.3–12.7)
Ethyl acetate	1.8	3.9	1.7	1.8	65.7±4.6
	(1.5–2.1)	(3.4–4.4)	(1.6–1.8)	(1.6–1.9)	(57.3–75.3)
Butanol	>100	>100	>100	>100	237.3±22.3
					(195.2 – 284.7)
Aqueous	>100	>100	>100	>100	712.5±39.9
					(636.2–792.6)
Doxorubicin	0.02	1.36	0.01	0.24	—
	(0.01–0.02)	(0.98–1.89)	(0.01–0.02)	(0.17–0.36)
Potassium dichromate	—	—	—	—	20

∗Values are expressed as mean ± S.E.M. Values in parenthesis represent 𝑃<0.05.