Development of a pyrG Mutant of Aspergillus oryzae Strain S1 as a Host for the Production of Heterologous Proteins

Table 1

Primers used in this study.

Designation	Primer description	Primer sequence
5′pyr	pyrG 5′ region sense primer	5′-GGCGCCACAATCGTTGAGGTA-3′
*revint-XhoI	pyrG 5′ region antisense primer, the XhoI site is underlined	5′-CTCGAGGTTGGCGATGGAGG-3′
*fwdint-XhoI	pyrG 3′ region sense primer, the XhoI site is underlined	5′-CTCGAGGTAGTGGTGGATACGTACT-3′
3′pyr	pyrG 3′ region antisense primer	5′-AAGCTTATCAGCTGCATATCTCTAT-3′
Pyr5′-out	pyrG 5′ region sense primer outside the pyrG 5′ gene replacement cassette	5′-ATTTAGTCATTCCTTTTGGCATC-3′
Pyr3′-out	pyrG 3′ region antisense primer outside the pyrG 3′ gene replacement cassette	5′-CTAATTTTAATGTCTTCCTTCAG-3′

∗revint-XhoI was used to amplify the targeting region downstream of the pyrG coding region.
*fwdint-XhoI was used to amplify the targeting region upstream of the pyrG coding region.