Stable Agrobacterium-Mediated Transformation of Maritime Pine Based on Kanamycin Selection

Table 1

Number of transformation events per gram of fresh mass in line L01 after 120 days on selective medium. LBA4404 strain did not show transformation events.

Strain	Cocultivation (hours)	OD600nm	Transformation events
AGL1	48	0.075	1.1 ± 0.2^e
AGL1	48	0.15	4.4 ± 0.7^cd
AGL1	48	0.3	7.7 ± 0.9^bc
AGL1	72	0.075	2.1 ± 0.6^de
AGL1	72	0.15	10.3 ± 1.2^ab
AGL1	72	0.3	11.4 ± 1.6^a
EHA105	48	0.075	0.0 ± 0.0^e
EHA105	48	0.15	0.0 ± 0.0^e
EHA105	48	0.3	0.7 ± 0.2^e
EHA105	72	0.075	0.0 ± 0.0^e
EHA105	72	0.15	0.7 ± 0.4^e
EHA105	72	0.3	0.9 ± 0.3^e

The data correspond to the mean values ± standard error in three independent experiments (𝑛=3). Different letters represent significant differences (Holm-Sidak, 𝛼=0.05).