Characterization of Cellulolytic Bacterial Cultures Grown in Different Substrates

Table 1

Production of cellulolytic enzymes from bacterial cultures under SSF for 4 and 7 days with different PKC : moisture ratios.

Bacterial culture	Moisture ratio	CMCase		Xylanase		Mannanase
		4 days	7 days	4 days	7 days	4 days	7 days
B. amyloliquefaciens DSMZ	1 : 0.2	3.91 ± 0.060^fgh	1.66 ± 0.045^ghi	3.32 ± 0.023^f	1.39 ± 0.082^fg	4.07 ± 0.030^f	2.08 ± 0.184^fgh
	1 : 0.3	3.72 ± 0.007^gh	1.12 ± 0.084^ij	3.20 ± 0.143^f	1.10 ± 0.045^ghi	3.83 ± 0.054^f	1.49 ± 0.126^hij
	1 : 0.4	3.29 ± 0.033^ghi	1.26 ± 0.096^hij	2.81 ± 0.032^f	1.15 ± 0.130^ghi	3.37 ± 0.042^fgh	1.48 ± 0.070^hij
	1 : 0.5	3.50 ± 0.012^gh	0.20 ± 0.047^mno	3.08 ± 0.018^f	0.49 ± 0.127^jklmn	3.58 ± 0.022^fg	0.89 ± 0.006^jkl
P. curdlanolyticus DSMZ	1 : 0.2	4.84 ± 2.300^efg	0.26 ± 0.150^mno	9.36 ± 0.360^c	0.25 ± 0.130^klmno	7.87 ± 2.100^d	0.78 ± 0.340^klm
	1 : 0.3	5.40 ± 0.440^def	0.10 ± 0.030^no	9.74 ± 0.070^c	0.13 ± 0.030^mno	4.82 ± 1.270^ef	0.78 ± 0.280^klm
	1 : 0.4	6.38 ± 0.310^d	2.16 ± 0.290^efg	9.76 ± 0.370^c	1.13 ± 0.280^ghi	3.43 ± 1.420^fgh	0.28 ± 0.140^mno
	1 : 0.5	10.40 ± 1.00^d	5.70 ± 0.030^c	14.35 ± 0.300^b	6.67 ± 0.750^b	8.82 ± 1.250^cd	5.35 ± 1.140^d
P. polymyxa ATCC	1 : 0.2	0.93 ± 0.112^lmno	2.50 ± 0.204^e	0.73 ± 0.006^ij	2.36 ± 0.161^de	0.80 ± 0.024^jkl	2.15 ± 0.112^fgh
	1 : 0.3	0.62 ± 0.014^mnop	0.91 ± 0.014^jkl	0.48 ± 0.015^jk	0.78 ± 0.002^ghij	0.71 ± 0.031^jkl	0.92 ± 0.074^jkl
	1 : 0.4	2.12 ± 0.303^ijk	2.53 ± 0.131^e	2.10 ± 0.044^g	2.10 ± 0.027^e	3.33 ± 0.580^fgh	2.77 ± 0.547^f
	1 : 0.5	6.45 ± 0.035^d	3.85 ± 0.110^d	6.30 ± 0.016^d	3.08 ± 0.300^cd	10.01 ± 0.363^cd	3.68 ± 0.083^e
B. megaterium ATCC	1 : 0.2	6.74 ± 0.570^d	3.46 ± 0.070^d	4.42 ± 0.080^e	3.53 ± 0.940^c	11.03 ± 3.760^c	4.10 ± 0.590^e
	1 : 0.3	5.82 ± 0.270^de	8.77 ± 0.220^b	4.98 ± 0.005^e	7.43 ± 0.030^b	7.11 ± 0.010^de	9.40 ± 0.090^c
	1 : 0.4	15.68 ± 0.230^b	12.76 ± 0.170^a	13.48 ± 0.020^b	10.80 ± 1.41^a	18.96 ± 0.440^b	13.91 ± 0.050^a
	1 : 0.5	31.24 ± 0.430^a	11.60 ± 0.110^a	25.34 ± 1.01^a	9.77 ± 0.260^a	35.99 ± 0.020^a	12.15 ± 0.090^b
P. glucanolyticus DSMZ	1 : 0.2	0.00 ± 0.000^p	0.00 ± 0.000^o	0.00 ± 0.000^m	0.00 ± 0.000^o	0.00 ± 0.000^l	0.00 ± 0.000^0
	1 : 0.3	0.00 ± 0.000^p	0.00 ± 0.000^o	0.00 ± 0.000^m	0.00 ± 0.000^o	0.00 ± 0.000^l	0.00 ± 0.000^o
	1 : 0.4	1.80 ± 0.220^jkl	0.28 ± 0.004^mno	1.38 ± 0.100^h	0.16 ± 0.008^mno	1.75 ± 0.080^hijk	0.33 ± 0.030^mno
	1 : 0.5	1.20 ± 0.060^klmno	1.00 ± 0.002^jkl	0.79 ± 0.050^ij	0.73 ± 0.002^hijk	1.17 ± 0.030^jk	1.12 ± 0.080^jk
C. fimi DSMZ	1 : 0.2	1.16 ± 0.160^klmno	0.51 ± 0.010^lmn	0.06 ± 0.030^lm	0.60 ± 0.250^ijklm	1.05 ± 0.040^jkl	1.94 ± 0.120^gh
	1 : 0.3	0.87 ± 0.260^lmno	0.98 ± 0.680^jkl	0.16 ± 0.040^lm	1.29 ± 0.130^fgh	1.80 ± 0.060^ghijk	0.67 ± 0.390^klmn
	1 : 0.4	1.35 ± 0.390^klmn	1.29 ± 0.030^hij	0.78 ± 0.290^ij	1.96 ± 0.060^ef	3.50 ± 0.360^fgh	2.48 ± 0.040^fg
	1 : 0.5	3.52 ± 0.030^gh	1.79 ± 0.280^fgh	1.80 ± 0.080^gh	2.10 ± 0.160^e	3.36 ± 0.100^fgh	1.83 ± 0.020^ghi
B.circulans ATCC	1 : 0.2	0.00 ± 0.000^p	0.00 ± 0.000^o	0.00 ± 0.000^m	0.00 ± 0.000^o	0.75 ± 0.500^jkl	0.00 ± 0.000^o
	1 : 0.3	0.47 ± 0.200^nop	0.38 ± 0.130^mno	0.70 ± 0.400^ij	0.00 ± 0.000^o	1.41 ± 0.090^ijk	0.05 ± 0.008^o
	1 : 0.4	0.55 ± 0.310^mnop	0.53 ± 0.070^klmn	0.94 ± 0.020^i	0.26 ± 0.120^jklmno	1.94 ± 0.130^ghij	0.20 ± 0.150^no
	1 : 0.5	2.78 ± 0.040^hij	0.60 ± 0.320^klm	1.47 ± 0.030^h	0.69 ± 0.020^hijkl	3.60 ± 0.610^fg	0.78 ± 0.080^klmn
B. wakoensis DSMZ	1 : 0.2	1.42 ± 0.080^klm	0.41 ± 0.270^mno	0.29 ± 0.060^kl	0.10 ± 0.020^mno	0.69 ± 0.030^jkl	0.29 ± 0.050^mno
	1 : 0.3	2.05 ± 0.020^ijk	2.40 ± 0.180^ef	0.56 ± 0.020^jk	0.26 ± 0.030^jklmno	1.28 ± 0.050^jk	1.18 ± 0.020^ijk
	1 : 0.4	1.63 ± 0.030^jkl	1.02 ± 0.020^jk	0.52 ± 0.040^jk	0.22 ± 0.003^klmno	0.97 ± 0.040^jkl	0.27 ± 0.040^mno
	1 : 0.5	2.17 ± 0.009^ijk	1.65 ± 0.020^ghi	0.77 ± 0.020^ij	0.03 ± 0.004^no	1.38 ± 0.020^ijk	0.99 ± 0.040^jk
B. cellulosilyticus DSMZ	1 : 0.2	0.00 ± 0.000^p	0.00 ± 0.000^o	0.00 ± 0.000^m	0.00 ± 0.000^o	0.00 ± 0.000^l	0.00 ± 0.000^o
	1 : 0.3	0.47 ± 0.002^nop	0.00 ± 0.000^o	0.32 ± 0.020^kl	0.00 ± 0.000^o	0.49 ± 0.030^kl	0.00 ± 0.000^o
	1 : 0.4	0.38 ± 0.030^op	0.00 ± 0.000^o	0.47 ± 0.050^jk	0.00 ± 0.000^o	0.73 ± 0.010^jkl	0.00 ± 0.000^o
	1 : 0.5	1.86 ± 0.160^jkl	0.38 ± 0.020^mno	0.78 ± 0.080^ij	0.21 ± 0.008^lkmno	3.00 ± 0.050^fghi	0.42 ± 0.002^lmno

a--pMeans ± SE. Means with different superscripts within the same column are significantly different (𝑃<0.05).