Characterization of Human Colorectal Cancer MDR1/P-gp Fab Antibody

Figure 4

Construction and verification of the P-gp recombinant. (a) The total RNA of human colorectal cancer, M: DL2000 DNA marker; lane 1: total RNA of human colorectal cancer. (b) PCR amplification of P-gp₂₁ gene, M: DL2000 DNA Marker; lane 1: blank control; lane 2: target gene of P-gp₂₁. (c) pET28a (+) and P-gp₂₁ gene digested by EcoR I/Xho I double enzyme, M: DL10000 DNA Marker; lane 1: the digested pET28a (+); lane 2: the digested P-gp₂₁ target gene. (d) Positive clone of P-gp₂₁ verified by double-enzyme cleavage, M: DL10000 DNA Marker; lane 1: positive clone of P-gp₂₁.

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