Characterization of Human Colorectal Cancer MDR1/P-gp Fab Antibody

Figure 7

Western blot analysis of clone number 29. (a) 1: primary antibody: crude cell extract of pComb3 clone; 2: primary antibody: crude cell extract of number 29 clone; 3: primary antibody: mouse anti-His antibody; 1, 2, and 3: antigen: P-gp₂₁ protein; secondary antibody: ALP-labeled horse anti-mouse IgG antibody. (b) 1: Antigen: BSA; 2: antigen: 15 kDa peptide; both 3 and 4: antigen: P-gp₂₁ protein; 1, 2, 3, and 4: primary antibody: purified Fab; secondary antibody: HRP-labeled goat anti-mouse IgG antibody.

(a)  

(b)