Characterization of Human Colorectal Cancer MDR1/P-gp Fab Antibody
Figure 9
Fab expression and purification. (a) SDS-PAGE: 1, pComb3 clone was induced by 0.5 mM IPTG at 28°C for 8 h; 2, number 29 was not induced; 3, number 29 was induced by 0.5 mM IPTG at 28°C for 6 h; 4, number 29 was induced by 0.5 mM IPTG at 28°C for 8 h. (b) SDS-PAGE: 1, sample before passing HiTrap Protein L column; 2, sample after passing HiTrap Protein L column; 3, the washout of the HiTrap Protein L column with binding buffer; 4, the washout of the HiTrap Protein L column with sodium acetate buffer (pH 2.3).
