Characterization of Thermo- and Detergent Stable Antigenic Glycosylated Cysteine Protease of Euphorbia nivulia Buch.-Ham. and Evaluation of Its Ecofriendly Applications

Table 1

Effect of surfactants, oxidizing agent, and metal ions on proteolytic activity.

Metal ions/O.A./Sur.	Concentration	Residual activity (%)
Control	Nil	100
SDS	1%	51.51 ± 0.18
H2O2	1%	68.18 ± 0.35
Tween 80	1%	100 ± 0.69
Triton X-100	1%	100 ± 1.04
KCl	5 mM	59.64 ± 0.09
NaCl	5 mM	64.97 ± 0.20
ZnCl2	5 mM	58.46 ± 0.16
AgSO4	5 mM	56.69 ± 0.04
CaCl2	5 mM	93.20 ± 0.16
CdCl2	5 mM	53.50 ± 0.29
HgCl2	5 mM	09.43 ± 0.57
FeCl3	5 mM	73.50 ± 0.17
MgCl2	5 mM	97.01 ± 0.22
MnCl2	5 mM	99.89 ± 0.45

Data represented in average values ± SD of 𝑛=6 experiment, O.A.: oxidizing agent; Sur: surfactants.