Authentication of Nigella sativa Seed Oil in Binary and Ternary Mixtures with Corn Oil and Soybean Oil Using FTIR Spectroscopy Coupled with Partial Least Square
Table 3
PLS performances at some frequency regions for the determination of NSO in binary mixtures with CO.
Frequencies region (cm−1)
Spectra
Calibration
Validation
RMSEC (% v/v)
RMSEP (% v/v)
4000–650
Normal
0.9818
3.50
0.9542
3.06
Derivative 1
0.9904
2.12
0.9975
1.32
Derivative 2
0.9968
1.61
0.9975
1.15
2977–3028, 1666–1739, 740–1446
Normal
0.9929
2.23
0.9849
2.80
Derivative 1
0.9933
1.85
0.9970
1.41
Derivative 2*
0.9984
1.34
0.9987
0.66
1666–1739
Normal
0.9926
2.48
0.9929
1.83
Derivative 1
0.9966
1.55
0.9950
1.88
Derivative 2
0.9915
2.08
0.9978
1.21
740–1446
Normal
0.9983
1.11
0.9965
1.34
Derivative 1
0.9983
1.19
0.9983
0.87
Derivative 2
0.9921
2.20
0.9952
1.82
*The frequency region and FTIR spectral treatment selected for quantification were marked with italics.