Research Article
HPLC-DAD-ESI/MS Identification of Light Harvesting and Light Screening Pigments in the Lake Sediments at Edmonson Point
Table 1
Pigments in Edmonson Point lake sediments identified using DAD and ESI mass spectra.
| Peak | Time (min) | Pigment | Main UV-vis bands (nm) | ESI+ () | ESI− () | Composition % |
| 1 | 3.1 | Reduced scytonemin | 385–450–590 | M+H 547.5 M+Na 569.5 |
[M−H]− 545.5 | 11.90 | 2 | 4.7 | Scytonemin | 385 | M+H 545.5 M+Na 567.5 |
[M−H]− 543.5 | 8.67 | 3 | 5.1 | Fucoxanthin | 440–470 | M+H 681 |
[M−H]− 657 | 9.92 | 4 | 5.9 | Lutein | 420–450–480 | M+H 591 |
[M−H]− 567 | 64.59 | 5 | 6.1 | Pheophytin b | (420) 450–475–665 | — |
[M−H]− 884 | 0.30 | 6 | 7.8 | Canthaxanthin | 478 | M+H 565 M+Na 587 |
[M−H]− 563 | 2.07 | 7 | 9.3 | Pheophytin a | 475–665 | — |
[M−H]− 870 | 0.70 | 8 | 12.1 | Chlorophyll b | 465–600–650 | — |
[M−H]− 965a | 0.65 | 9 | 15.1 | Bacteriochlorophyll-c | 370–420–610–660 | — |
[M−H]− 870a | 0.24 | 10 | 19.2 | Chlorophyll a | 370–415–435–610–660 | — |
[M−H]− 951a | 0.30 |
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Acetone adduct.
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