HPLC-DAD-ESI/MS Identification of Light Harvesting and Light Screening Pigments in the Lake Sediments at Edmonson Point

Table 1

Pigments in Edmonson Point lake sediments identified using DAD and ESI mass spectra.

Peak	Time (min)	Pigment	Main UV-vis bands (nm)	ESI+ (𝑚/𝑧)	ESI− (𝑚/𝑧)	Composition %
1	3.1	Reduced scytonemin	385–450–590	[M+H]^+ 547.5; [M+Na]^+ 569.5	[M−H]^− 545.5	11.90
2	4.7	Scytonemin	385	[M+H]^+ 545.5; [M+Na]^+ 567.5	[M−H]^− 543.5	8.67
3	5.1	Fucoxanthin	440–470	[M+H]^+ 681	[M−H]^− 657	9.92
4	5.9	Lutein	420–450–480	[M+H]^+ 591	[M−H]^− 567	64.59
5	6.1	Pheophytin b	(420) 450–475–665	—	[M−H]^− 884	0.30
6	7.8	Canthaxanthin	478	[M+H]^+ 565; [M+Na]^+ 587	[M−H]^− 563	2.07
7	9.3	Pheophytin a	475–665	—	[M−H]^− 870	0.70
8	12.1	Chlorophyll b	465–600–650	—	[M−H]^− 965^a	0.65
9	15.1	Bacteriochlorophyll-c	370–420–610–660	—	[M−H]^− 870^a	0.24
10	19.2	Chlorophyll a	370–415–435–610–660	—	[M−H]^− 951^a	0.30

aAcetone adduct.