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The Scientific World Journal
Volume 2013 (2013), Article ID 746254, 5 pages
Research Article

The Aquatic Environment as a Reservoir of Vibrio cholerae O1 in Hydrographic Basins of the State of Pernambuco, Brazil

1Centro de Pesquisas Aggeu Magalhães, FIOCRUZ-PE, Avendia Professor Moraes Rego, s/n. Cidade Universitária, 50670-420 Recife, PE, Brazil
2Laboratório Central de Saúde Pública Dr. Milton Bezerra Sobral (LACEN-PE), Secretaria Estadual de Saúde de Pernambuco (SES-PE), Praça Oswaldo Cruz, s/n. Boa Vista, 50050-911 Recife, PE, Brazil
3Instituto Oswaldo Cruz (IOC), FIOCRUZ-RJ, Avenida Brasil, 4365 Manguinhos, 21040-360 Rio de Janeiro, RJ, Brazil

Received 6 December 2012; Accepted 23 January 2013

Academic Editors: K. Ohmiya, J. L. Romalde, and D. Zhou

Copyright © 2013 Carina Lucena Mendes-Marques et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


After the worldwide cholera epidemic in 1993, permanent environmental monitoring of hydrographic basins was established in Pernambuco, Brazil, where cholera is endemic. After a quiescent period, 4 rfbN (serogroup O1) positive water samples that were culture negative were detected by multiplex single-tube nested PCR (MSTNPCR); 2 of these were also ctxA (cholera toxin) positive. From May to June 2012, 30 V. cholerae O1 isolates were obtained by culturing samples. These isolates were analyzed for the presence of virulence genes by PCR, intergenic spacer region 16S-23S PCR (ISR-PCR), and pulsed field gel electrophoresis (PFGE). The isolates were positive for the rfbN gene and negative for the assessed pathogenic genes and were classified into 2 groups by ISR and the same profile by PFGE. Close genetic similarity was observed between them (2012) and environmental strains from 2004 to 2005, indicating the permanence of endemic V. cholerae O1 in the region.