The Determination of Six Ionophore Coccidiostats in Feed by Liquid Chromatography with Postcolumn Derivatisation and Spectrofotometric/Fluorescence Detection

Figure 2

The comparison of the separation of six ionophore coccidiostats with traditional porous column and fused core technology. (a) Chromatogram obtained on Phenomenex Luna C18(2) column (150×4.6 mm, 3 μm) with gradient elution of methanol (A) and 0.02 M KH₂PO₄ (B) 0–10 min: 88% A, 14–19 min: 90% A, 22–32 min: 88% A; flow rate 0.7 mL/min. (b) Chromatogram obtained on Phenomenex Kinetex C18 column (150×4.6 mm, 2.6 μm) with isocratic elution with methanol and 0.02 M KH₂PO₄ (88 : 12, v : v); flow rate 0.7 mL/min.

(a)

(b)