In Vitro Activation and Inhibition of Recombinant EGFR Tyrosine Kinase Expressed in Escherichia coli
Figure 2
Dimerization and tyrosine kinase assays. Total protein extracts of E. coli transformed by pGEX-TKJMΔ (Δ) were treated with BS3 or glutaraldehyde (GLU) as indicated and analyzed by western blot with the anti-GST antibody (a). A control (C) without cross-linkage agent is also shown. The positions of the protein size marker (Bio-Rad) are indicated from 30 to 97 kD. The recombinant proteins were assayed with ATP on immobilized glutathione-Sepharose beads as described in Section 2 (b). The empty pGEX-6P plasmid was used as control (P). Genistein (Genist) was added where indicated.
