In Vitro Activation and Inhibition of Recombinant EGFR Tyrosine Kinase Expressed in Escherichia coli

Figure 3

Recombinant protein purification. The recombinant GST-TKJMΔ and GST proteins were purified as described in Section 2. Lane 1: flow through; lane 2: ATP-wash; and lane 3: eluted proteins. The samples were run in 10% SDS/PAGE and stained with Coomassie blue. The arrow points to the TKJMΔ recombinant protein.