Application of Response Surface Methodology for Optimizing Arginine Deiminase Production Medium for Enterococcus faecium sp. GR7

Table 2

Experimental design and results of central composite design for ADI specific activity and growth from E. faecium sp. GR7.

Run	Tryptone	Lactose	Arginine	Growth (𝐴600)		Specific activity (IU/mG)
	(g/L)	(g/L)	(g/L)	Predicted value	Experimental value	Predicted value	Experimental value
1	0	0	0	1.771	1.156	0.001	0.839
2	0	−α	0	1.822	0.103	1.153	0.172
3	0	0	+α	0.328	0.185	2.103	0.888
4	−1	−1	−1	1.706	0.002	0.083	0.0002
5	+1	+1	+1	0.029	1.607	3.332	0.643
6	−1	+1	−1	1.232	0.054	0.835	4.169
7	−1	−1	+1	1.232	0.022	0.835	2.406
8	+1	+1	−1	−0.102	1.377	1.906	0.251
9	0	0	0	1.232	1.249	0.835	0.839
10	+1	−1	−1	0.237	0.259	2.103	0.295
11	0	0	0	0.548	1.156	0.261	0.839
12	0	0	0	1.232	1.249	0.835	1.056
13	0	0	0	1.232	1.26	0.835	0.779
14	+α	0	0	0.149	2.26	0.761	0.492
15	0	+α	0	1.232	1.276	1.743	1.272
16	+1	0	+1	0.322	0.154	4.295	4.274
17	+1	−1	+1	0.029	0.028	3.399	4.034
18	0	0	0	0.173	1.26	−0.189	0.839
19	−α	0	0	1.068	0.041	1.743	3.109
20	0	0	−α	0.097	0.958	3.508	0.428