Gene Therapy with HSV1-sr39TK/GCV Exhibits a Stronger Therapeutic Efficacy Than HSV1-TK/GCV in Rat C6 Glioma Cells
Figure 4
Propidium iodide (PI) and Hoechst 33342 double staining in cultured C6 cells 72 hours after 400 μM ganciclovir (GCV) administration (×400). Cells emitting blue fluorescence were Hoechst positive while those with red fluorescence were PI positive. Although PI positive cells (dead cells) were rare in control group (a), their number increased significantly in C6-TK (b) and C6-sr39TK group (c) (C6-TK versus C6-sr39TK, ).
