Statistical Optimization of Process Parameters for Lipase-Catalyzed Synthesis of Triethanolamine-Based Esterquats Using Response Surface Methodology in 2-Liter Bioreactor

Table 6

Optimum conditions derived by RSM for synthesis of TEA-based esterquat.

Methods	Optimal conditions					Conversion %
	Enzyme amount (w/w%)	Reaction time (h)	Reaction temperature (°C)	Molar ratio of substrates (mole)	Agitation speed (r.p.m.)	Actual	Predicted	RSE%
RSM	4.77	24	61.9	1 : 1	480	63.57	65.08	2.32
ANN (QP)	4.77	24	61.9	1 : 1	480	63.57	61.14	3.98