Research Article

Agrobacterium tumefaciens-Mediated Transformation of Valsa mali: An Efficient Tool for Random Insertion Mutagenesis

Figure 3

Genetic stability analysis of transformants on PDA medium containing 50 ug/mL hygromycin B after subculturing for five generations on PDA plates without antibiotics. Three independent transformants (PFL1, PFL4, and PFL6) exhibit normal growth on hygromycin B containing medium and wild-type strain LXS240101 (W) shows no growth. The plates were incubated at 25°C for 48 hours.
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