The Scientific World Journal

Research Article

Molecular Detection of Porcine Torovirus in Piglets with Diarrhea in Southwest China

Table 1

RT-PCR and nested PCR primers used in this study.


Target viruses^a	Target genes^b	Primer sequence, 5′–3^′c	Size (bp)	Source or reference

PToV	S	F: ACCCCTGCCTGAGGTTTCYTT R: AGCACGACGTTGTCTRCGTGT	451	Established by our own laboratory
PEDV	S	F: TTCTGAGTCACGAACAGCCA R: CATATGCAGCCTGCTCTGAA	651	Kim et al. (2001) [11]
PKBV	3D	F: TGGATTACAAGTGTTTTGATGC R: TGTCGTAGAACTCCTTGATGAA	313	Established by our own laboratory
PRV A	VP6	F: AAAGATGCTAGGGACAAAATTG R: TTCAGATTGTGGAGCTATTCCA	308	Elschner et al. (2002) [12]
PRV A	VP6	nF: GACAAAATTGTCGAAGGCACATTATA nR: TCGGTAGATTACCAATTCCTCCAG	121	Elschner et al. (2002) [12]
PRV B	NSP2	F: CTATTCAGTGTGTCGTGAGAGG R: GCAGACAAGCTAGCCCGCTTCG	434	Gouvea et al. (1991) [13]
PRV C	VP6	F: CTCGATGCTACTACAGAATCAG R: AGCCACATAGTTCACATTTCATCC	366	Gouvea et al. (1991) [13]
PRV C	VP6	nF: CTCGATGCTACTACAGAATCAG nR: GGGATCATCCACGTCATGCGT	328	Gouvea et al. (1991) [13]
TGEV	ORF1b	F: GGGTAAGTTGCTCATTAGAAATAATGG R: CTTCTTCAAAGCTAGGGACTG	1006	Kim et al. (2010) [14]
TGEV	S	F: GGGTAAGTTGCTCATTAGAAATAATGG R: CTTCTTCAAAGCTAGGGACTG	1006	Kim et al. (2010) [14]
AV	RdRp	F11: GARTTYGATTGGRCKCGKTAYGA F12: GARTTYGATTGGRCKAGGTAYGA F21: CGKTAYGATGGKACKATHC F22: AGGTAYGATGGKACKATHC R1: GGYTTKACCCACATICCRAA	422	Chu et al. (2008) [15]
MRV	L1	F: GCATCCATTGTAAATGACGAGTCTG R: CTTGAGATTAGCTCTAGCATCTTCTG	416	Decaro et al. (2005) [16]
MRV	L1	nF: GCTAGGCCGATATCGGGAATGCAG nR: GTCTCACTATTCACCTTACCAGCAG	344	Decaro et al. (2005) [16]
PSaV and PNoV	RdRp	F: GATTACTCCAAGTGGGACTCCAC R: TGACAATGTAATATCACCATA	319	Kim et al. (2010) [14]

PEDV: porcine epidemic diarrhea coronavirus; PKBV: porcine kobuvirus; PRV A–C: porcine group A–C rotaviruses; TGEV: transmissible gastroenteritis coronavirus; AV: astroviruses; MRV: mammalian orthoreovirus; PSaV: porcine sapovirus; PNoV: porcine norovirus.
ORF: S: spike protein; 3D: RNA dependent RNA polymerase; VP6: viral protein 6; NSP2: nonstructural protein 2; ORF1b: open reading frame 1b; L1: large segment 1; RdRp: RNA dependent RNA polymerase.
F: upstream primer for RT-PCR; R: downstream primer for RT-PCR; nF: upstream primer for nested PCR; nR: downstream primer for nested PCR.