Validation of a Thin-Layer Chromatography for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation
Figure 1
(a) Densitogram of the combine standard solution containing the following reference substances: lidocaine hydrochloride (L), hydrocortisone acetate (HA) and its related substances, namely, hydrocortisone (H), prednisolone (PR), and cortisone acetate (CRA) and (b) densitogram of drug sample analyzed by NP-TLC, at wavelengths of 200 nm and 250 nm obtained under optimum chromatographic conditions.