Research Article

Validation of a Thin-Layer Chromatography for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation

Table 1

Experimental design matrix (23) for robustness test for biological active substances coming from injection dosage form of examined drug.

Experiment no. Biological active substancea content ( ) [mg] in examined drug
HAL

1+++++++123.724.8
2+++119.525.9
3+++124.325.6
4+++121.424.4
5+++126.024.2
6+++123.924.5
7+++120.124.6
8+++120.825.6

Effect for HA, L in examined drug
 HA−0.4751.6252.125−2.8751.0251.425−0.375
 L0.450 −0.200−0.3000.550−0.4000.350−0.750

HA: hydrocortisone acetate; L: lidocaine hydrochloride.
: sorbent type; : chamber type; : temperature of plate activation; : distance of development; : saturation time of the chamber; : volume of chloroform; : volume of acetone.