ErbB4 as a Potential Molecular Target in the Treatment of Esophageal Squamous Cell Cancers

Figure 4

Analysis of effect of miR-140-5p on EC9706 cells via flow cytometer after staining with Annexin V (a). Percentage cell apoptosis was examined using flow cytometer after transfecting EC9706 and TE-1 cells with miR-140-5p (b and c). The percentage of cells in early and late apoptosis was quantified by interpretation of quadrant (UR and LR) with regard to control. The apoptosis assay was performed by staining Annexin V/PI on cells and analyzed by flow cytometry (d). The expression of apoptotic-related proteins, cleaved caspase 3, and cleaved PARP-1 was detected by western blot analysis, and β-actin was used as a loading control.

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