The Genetic Structure of Wild Orobanche cumana Wallr. (Orobanchaceae) Populations in Eastern Bulgaria Reflects Introgressions from Weedy Populations
Table 2
Genetic diversity parameters of Orobanche cumana populations from Bulgaria collected on wild hosts and on sunflower (prefix CUMBUL-) and from Spain collected on sunflower (prefix IASCum-).
Population
Na (±SE)
Na ≥ 5% (±SE)
Ne (±SE)
Npa (±SE)
Ho (±SE)
He (±SE)
(±SE)
Pairwise differences
Genotypic richness
(±SE)
O. cumana populations collected on wild hosts
CUMBUL-1
66.7
1.733 (0.15)
1.667 (0.13)
1.391 (0.10)
0.000 (0.00)
0.004 (0.01)
0.229 (0.05)
0.349 (0.08)
3.111 (1.66)
7
0.40
0.984 (0.01)
0.992
CUMBUL-2
86.9
2.000 (0.14)
1.933 (0.15)
1.521 (0.10)
0.000 (0.00)
0.000 (0.00)
0.297 (0.05)
0.458 (0.07)
4.393 (2.20)
8
0.24
1.000 (0.00)
1.000
CUMBUL-3
0.0
1.000 (0.00)
1.000 (0.00)
1.000 (0.00)
0.000 (0.00)
0.000 (0.00)
0.000 (0.00)
0.000 (0.00)
0.000 (0.00)
1
0.00
—
—
CUMBUL-4
53.3
1.600 (0.16)
1.333 (0.13)
1.145 (0.05)
0.067 (0.07)
0.000 (0.00)
0.105 (0.03)
0.184 (0.06)
1.595 (0.96)
6
0.18
1.000 (0.00)
1.000
CUMBUL-5_1
80.0
2.000 (0.17)
1.867 (0.16)
1.250 (0.07)
0.000 (0.00)
0.032 (0.01)
0.171 (0.04)
0.306 (0.06)
2.449 (1.34)
11
0.37
0.776 (0.04)
0.874
CUMBUL-5_2
80.0
1.933 (0.15)
1.933 (0.15)
1.418 (0.10)
0.000 (0.00)
0.014 (0.01)
0.248 (0.05)
0.400 (0.07)
3.597 (1.86)
7
0.32
0.920 (0.04)
0.958
CUMBUL-6
73.3
1.800 (0.14)
1.667 (0.16)
1.294 (0.10)
0.000 (0.00)
0.003 (0.01)
0.181 (0.05)
0.300 (0.07)
2.629 (1.43)
10
0.41
0.968 (0.03)
0.984
CUMBUL-7
73.3
1.800 (0.14)
1.800 (0.14)
1.467 (0.12)
0.000 (0.00)
0.010 (0.01)
0.258 (0.05)
0.398 (0.08)
3.947 (2.04)
7
0.46
0.975 (0.01)
0.987
Mean
64.2
1.733 (0.06)
1.650 (0.12)
1.311 (0.03)
0.008 (0.01)
0.008 (0.002)
0.186 (0.02)
0.299 (0.03)
2.715 (0.05)
7.1
0.30
0.946
0.971
O. cumana populations collected on sunflower
CUMBUL-8
40.0
1.400 (0.13)
1.133 (0.09)
1.039 (0.02)
0.000 (0.00)
0.021 (0.01)
0.034 (0.01)
0.071 (0.03)
0.446 (0.41)
3
0.11
0.194 (0.10)
0.325
CUMBUL-9
13.3
1.133 (0.09)
1.133 (0.09)
1.014 (0.01)
0.000 (0.00)
0.000 (0.00)
0.013 (0.01)
0.026 (0.02)
0.195 (0.25)
2
0.05
1.000 (0.00)
1.000
CUMBUL-10
46.7
1.467 (0.13)
1.467 (0.13)
1.175 (0.06)
0.000 (0.00)
0.015 (0.01)
0.123 (0.04)
0.201 (0.06)
1.825 (1.07)
8
0.41
0.915 (0.04)
0.956
Mean-Bulgaria
33.3
1.333 (0.10)
1.244 (0.11)
1.076 (0.05)
0.000 (0.00)
0.012 (0.01)
0.057 (0.03)
0.099 (0.05)
0.822 (0.51)
4.3
0.19
0.703
0.760
IASCum-2
0.0
1.000 (0.00)
1.000 (0.00)
1.000 (0.00)
0.000 (0.00)
0.000 (0.00)
0.000 (0.00)
0.000 (0.00)
0.000 (0.00)
1
0.00
—
—
IASCum-3
0.0
1.000 (0.00)
1.000 (0.00)
1.000 (0.00)
0.000 (0.00)
0.000 (0.00)
0.000 (0.00)
0.000 (0.00)
0.000 (0.00)
1
0.00
—
—
IASCum-4
6.7
1.067 (0.07)
1.067 (0.07)
1.026 (0.03)
0.000 (0.00)
0.000 (0.00)
0.019 (0.02)
0.030 (0.03)
0.290 (0.32)
2
0.09
1.000 (0.02)
1.000
Mean-Spain
2.2
1.022 (0.02)
1.022 (0.02)
1.009 (0.01)
0.000 (0.00)
0.000 (0.00)
0.006 (0.01)
0.010 (0.01)
0.097 (0.10)
0.7
0.03
: percentage of polymorphic loci, Na: average observed allele number, Na > 5%: number of different alleles with a frequency ≥ 5%, Ne: number of effective alleles, Npa: number of private alleles unique to a single population, Ho: observed heterozygosity, He: expected heterozygosity, : Shannon’s diversity index, Pairwise differences: mean number of pairwise differences between individuals within each population (±SD), : number of distinct multilocus genotypes (MLGs), : genotypic richness; : fixation index, and : selfing rate.
