Immunomodulatory Effects of Hemagglutinin- (HA-) Modified A20 B-Cell Lymphoma Expanded as a Brain Tumor on Adoptively Transferred HA-Specific CD4+ T Cells
Figure 1
A20HA brain tumor growth in syngeneic BALB/c mice leads to fast death of mice and fatal depletion of spleens and lymph nodes in symptomatic mice. (a) Kaplan-Meier survival curve showing growth rates of A20HA B-cell lymphoma in brain after i.c. injection of 10,000 and 50,000 tumor cells ( in each group). A representative experiment among the three is shown. (b) Photomicrographs of eosin and hematoxylin stained coronal sections of the brains, spleens, and lymph nodes from (a) on day 21 after A20HA i.c. inoculation showing the forming of brain tumors (showed by arrows) and depletion of spleens and lymph nodes in asymptomatic (As) and symptomatic (Sy) mice that received 10,000 and 50,000 tumor cells, correspondingly. Magnifications ×1 and ×40. (c) RT-PCR of spleens and lymph nodes showing minor metastases of A20HA cells in lymph nodes on day 14 after i.c. A20HA injection (50,000 cells). Three individual mice in each group were analyzed. .
