Immunomodulatory Effects of Hemagglutinin- (HA-) Modified A20 B-Cell Lymphoma Expanded as a Brain Tumor on Adoptively Transferred HA-Specific CD4+ T Cells
Figure 5
Proliferative response and production of IFN-γ by HA-specific CD4+Thy1.1+ T cells from A20HA brain tumor-bearing mice demonstrate nonanergic status of a part the adoptively transferred Th cells. HA-specific CD4+Thy1.1+ T cells were injected i.v. on day 5 after i.c. tumor inoculation into A20HA brain tumor-bearing mice (A20HA + T cells) and tumor-free control mice (T cells). A20HA brain tumor-bearing mice in the absence of HA-specific CD4+Thy1.1+ T cells represented a negative control (A20HA). Spleens and cervical lymph nodes were pooled and analyzed after 72 h incubation with or without HA peptide in triplicates. (a) Proliferative response of HA-specific CD4+ T cells from spleens of A20HA brain tumor-bearing mice to restimulation in vitro with HA peptide is not reduced. Cell proliferation was measured by [3H]TdR incorporation on day 16 after the adoptive transfer. (b) Production of IFN-γ by HA-specific CD4+ T cells from cervical lymph nodes of A20HA brain tumor-bearing mice induced by restimulation in vitro with HA peptide is maximal on day 9 and reduced on day 16 after the adoptive transfer. IFN-γ concentration was measured by ELISA. Data are represented as the mean ± SD, . .
