An Analysis of the Malassezia Species Distribution in the Skin of Patients with Pityriasis Versicolor in Chengdu, China

Table 1

Universal fungal primer PCR reaction system.

Components	Sample amount
PCR reaction mixture	12.5 L (including 2.5 U Pfu Taq enzyme, 500 M dNTP, 20 mM Tris-Cl (pH 8.3), 100 mM KCl, and 3 mM MgCl2)
ITS1^a	1 L
ITS4^b	1 L
Template DNA	2 L
Double distilled water	8.5 L

Internal transcribed spacer 1; ^binternal transcribed spacer 4.