Research Article
An Analysis of the Malassezia Species Distribution in the Skin of Patients with Pityriasis Versicolor in Chengdu, China
Table 3
Reaction system for real-time fluorescence quantitative PCR.
| | Components | Sample amount |
| | 10 × buffer (without Mg2+) | 3 L | | MgCl2 (25 mM) | 3 L | | dNTP (25 mM) | 0.36 L | | Sense prime (10 M) | 1 L | | Antisense primer (10 M) | 1 L | | TaqMan probe (10 M) | 0.6 L | | Taq enzyme (5 U/L) | 0.3 L | | Double distilled water | 18.74 L | | Template DNA | 2 L |
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