Pharmacodynamics of TRPV1 Agonists in a Bioassay Using Human PC-3 Cells
Figure 1
Calcium imaging data analysis. The regions of interest (ROIs) of 10 cells per plate (colored ellipsis) are marked to measure changes in fluorescence and a ROI without cells (dark red rectangle) is also marked to measure the background signal (a). Increases in fluorescence are plotted as a time function for each cell. The increase of fluorescence () is calculated as the difference between the mean of the peak and 2 frames backward and forward (narrow rectangle measurements) and the mean of the measurements of 10 frames before agonist exposition (wide rectangle of measurements); the background signal (red line) is then subtracted (b).
