Pharmacodynamics of TRPV1 Agonists in a Bioassay Using Human PC-3 Cells
Figure 2
Calcium transients and dose-response curves. The means of measurements for each agonist concentration are plotted as time functions of the increase in fluorescence related to the signal before exposition (a, c, and e); ionomycin was applied at the end of each experiment and its maximum effect was used to normalize the effect of the different agonists and concentrations. The slope showing calcium entrance induced by capsaicin and piperine exposition (capsaicin 830.1 ± 128.1 relative increase of fluorescence per second and piperine 1238 ± 146.8 relative increase of fluorescence per second) (a, c) is sharper than the slope showing calcium entrance induced by natural capsaicinoids exposition (184.6 ± 17.39 relative increase of fluorescence per second) (e). The normalized effect of each concentration is plotted as a dose-response curve for each agonist. Each point represents the mean ± SEM of 3 independent experiments () (b, d, and f).
