Molecular Phylogeny and Predicted 3D Structure of Plant beta-D--Acetylhexosaminidase
Figure 6
The molecular 3D modeling of tomato beta--acetyl hexosaminidase (-Hex-Sl). SPDB viewer and Chimera were used to prepare the images. (a) The predicted 3D modeled structure is shown as ribbon diagram. The structure contains two fold domains (I and II) including -helix (red), -pleated sheets (purple), and coils (gray) The catalytic domain II is a (/)8 barrel with the active site located at the C terminus of the barrel. Template used for building this structure was 1now_B(PDB). (b) Superimposition magic fit image of the modeled structure -Hex-Sl (blue) with template structure human 1now, human --acetyl-hexosaminidase (red), and human -hexosaminidase B-subunit. (c) The predicted ligand-binding site (active site) residues identified are depicted by as blue color. (d) Space filled view of ligand biding site of -Hex-Sl with docking substrate -acetyl--D-glucosamine (NAG).
