Genetic Variability and Phylogeny of Current Chinese Porcine Epidemic Diarrhea Virus Strains Based on Spike, ORF3, and Membrane Genes

Table 2

Primers used in RT-PCR for PEDV spike, ORF3, and membrane genes.

Primer	Nucleotide sequence (5′-3′)	Size of product (bp)
S-F	TTCTGAGTCACGAACAGCCA	651
S-R	CATATGCAGCCTGCTCTGAA
ORF3-F	TCCTAGACTTCAACCTTACG	833
ORF3-R	GGTGACAAGTGAAGCACAGA
M-F	GTCTTACATGCGAATTGACC	808
M-R	GGCATAGAGAGATAATGGCA