The Scientific World Journal

Research Article

Diverse Effects of Lead Nitrate on the Proliferation, Differentiation, and Gene Expression of Stem Cells Isolated from a Dental Origin

Table 2

Immunophenotype analysis of bone marrow mesenchymal stem cells (BM-MSCs), dental permanent stem cells (DPSCs), dental deciduous stem cells (SCDs), and periodontal ligament stem cells (PDLs), respectively, cultured before and after treatment of Pb²⁺. BM-MSCs were tested against human antigens CD34, CD44, CD45, CD73, CD166, and HLA-DR. 7-AAD was used to check the viability of the cells. The results represent average of 3 independent culture replicates.

(a)


BM-MSCs	7AAD	HLA-DR	CD34	CD45	CD44	CD73	CD90	CD166

Control	9.78%	20.22%	20.25%	21.81%	77.04%	74.99%	72.78%	51.50%
10 μM	2.60%	17.32%	18.74%	18.34%	80.86%	74.48%	70.36%	46.44%
20 μM	7.60%	25.24%	25.90%	23.34%	74.48%	67.78%	62.44%	54.72%
40 μM	6.24%	17.94%	16.06%	18.66%	82.24%	77.70%	68.99%	57.22%
80 μM	7.40%	20.89%	22.57%	21.54%	73.09%	74.27%	66.16%	67.62%
160 μM	10.78%	24.89%	27.07%	26.32%	70.62%	68.45%	52.87%	68.19%

(b)


DPSCs	7AAD	HLA-DR	CD34	CD45	CD44	CD73	CD90	CD166

Control	2.82%	24.21%	23.50%	23.94%	74.62%	76.49%	48.87%	53.03%
10 μM	3.86%	20.11%	19.80%	20.16%	80.58%	80.72%	66.41%	69.32%
20 μM	6.59%	30%	27.30%	28.49%	60.33%	74.84%	65.98%	71.66%
40 μM	6.03%	30.64%	31.68%	31.32%	69.44%	66.48%	65.23%	67.20%
80 μM	4.82%	33.42%	31.90%	32.63%	78.31%	69.22%	70.07%	66.02%
160 μM	4.73%	31.04	32.05%	31.87%	66.45%	67.93%	60.14%	68.05%

(c)


SCDs	7AAD	HLA-DR	CD34	CD45	CD44	CD73	CD90	CD166

Control	3.28%	19.82%	21.88%	21.71%	74.75%	75.40%	68.23%	47.84%
10 μM	3.57%	21.88%	28.57%	27.57%	55.71%	69.13%	59.82%	53.25%
20 μM	49.95%	30.18%	34.44%	30.51%	70.16%	72.23%	75.66%	41.45%
40 μM	45.16%	43.36%	42.06%	42.06%	60.97%	65.94%	62.75%	41.83%
80 μM	43.90%	50.52%	49.71%	49.60%	36.82%	48.71%	52.05%	30.39%
160 μM	47.17%	44.86%	45.07%	45.20%	55.93%	63.16%	60.94%	41.94%

(d)


PDLs	7AAD	HLA-DR	CD34	CD45	CD44	CD73	CD90	CD166

Control	7.74%	15.60%	15.57%	15.32%	78.33%	80.23%	77.15%	44.02%
10 μM	2.66%	23.34%	23.23%	24.05%	72.54%	73.31%	71.62%	53.57%
20 μM	3.48%	28.70%	28.24%	29.23%	66.42%	69.62%	62.05%	34.90%
40 μM	3.01%	30.84%	31.68%	31.70%	62.60%	64.96%	58.66%	34.13%
80 μM	5.79%	31.34%	33.75%	34.65%	60.92%	60.24%	58.84%	42.02%
160 μM	7.65%	39.17%	38.43%	38.99%	55.80%	58.91%	70.07%	45.62%