Evaluation of Different Culture Media for Improvement in Bioinsecticides Production by Indigenous Bacillus thuringiensis and Their Application against Larvae of Aedes aegypti

Table 1

Biomass production of Bt  𝑠𝑣2 in different carbon sources.

Sugar source (g/L)	SC (g/L)	Biomass (g/L)	𝑌 coefficient
BC + glucose (10)	4.70 (0.02)^e	6.30 (0.03)^a	1.34
BC + fructose (10)	7.12 (0.04)^b	4.30 (0.09)^c	0.60
BC + mannitol (10)	8.02 (0.03)^a	1.27 (0.05)^e	0.14
BC + sucrose (10)	5.28 (0.02)^d	3.66 (0.07)^d	0.70
BC + starch (10)	7.93 (0.02)^a	1.07 (0.02)^e	0.13
BC + banana (10)	4.82 (0.03)^e	5.87 (0.08)^b	1.22
BC + beet root (10)	6.32 (0.08)^c	5.10 (0.11)^b	0.80
BC + mahua (10)	6.76 (0.04)^c	3.61 (0.04)^d	0.53
LB medium	—	5.40 (0.03)^b	—

SC: Sugar consumed at the end of fermentation; initial sugar concentration used in each test medium = 10 gm/L; BC: basal medium for carbon source study (NaCl 2.5 gm/L + Na₂HPO₄ 1 gm/L + MgSO₄ 0.2 gm/L + MnCl₂ 0.05 gm/L + yeast extract 5 gm/L). Yield (𝑌) coefficient expressed as the ratio of carbon in the newly formed biomass to the carbon in the respective sugar source.
Data are presented as mean (SD).
Means within a given column followed by the same letter are not significantly different, Tukey’s MRT (𝛼=0.05), 𝑃<0.0001.