Determination of Suitable Microspore Stage and Callus Induction from Anthers of Kenaf (Hibiscus cannabinus L.)

Table 3

Effect of the different types of PGR on callus induction.

Number of treat	NAA mg/L	2,4-D mg/L	KIN mg/L	6-BA mg/L	Number of anthers inoculated	Average number of anthers responded	Callus formation frequency %
T1	2.0		1.0	—	60	3.0^de	30
T2	2.0		2.0	—	60	2.5^ef	25
T3	3.0		1.0	—	60	4.5^cd	45
T4	3.0		2.0	—	60	5.0^c	50
T5	2.0		—	2.0	60	7.3^ab	73
T6	2.0		—	3.0	60	6.2^bc	62
T7	3.0		—	2.0	60	8.0^a	80
T8	3.0		—	3.0	60	9.0^a	90
T9		1.0	1.0	—	60	2.0^ef	20
T10		1.0	2.0	—	60	1.3^ef	13
T11		2.0	1.0	—	60	1.0^f	10
T12		2.0	2.0	—	60	3.0^de	30
T13		1.0	—	2.0	60	2.5^ef	25
T14		1.0	—	3.0	60	2.2^ef	22
T15		2.0	—	2.0	60	3.0^de	30
T16		2.0	—	3.0	60	2.5^ef	25

Values followed by the same letters within a column are not significantly different (𝑃≤0.05); Duncan’s Multiple Range Test (DMRT).