Conjugates of a Photoactivated Rhodamine with Biopolymers for Cell Staining

Figure 3

Microphotographs of the A431 cells stained by PFD (novel dye called Rho813 after photoactivation) and the standard dyes MitoTracker Green FM and Hoechst 33342. (a) Image obtained with 3 channels (for each dye) simultaneously. (b) The cell image obtained only with green channel (MitoTracker Green FM). (c) The cell image obtained only with red channel (Rho813). (d) The cell image obtained with overlapping green and red channels that gave white spots (“colocalization” areas of red and green fluorescence).

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