Effect of Fluorosis on Liver Cells of VC Deficient and Wild Type Mice
Figure 2
Effects of NaF on antioxidant status. -axis: cells from different mice in treatment. -axis: units of enzyme activity. (a) SOD activity was measured using the SOD determination kit. The SOD activity was calculated as an inhibition rate percentage and expressed as units (SOD activity (units) = SOD activity (inhibition rate))/(1-SOD activity (inhibition rate)). (b) CAT activity was determined using the OxiSelect Catalase Activity assay kit. One unit of CAT activity is equal to the amount of enzyme(s) that decomposed 1.0 μmole of H2O2 per minute at 25°C. The CAT activity was calculated and expressed as per mg protein. (c) GPx activity was measured with the Glutathione Peroxidase assay kit. The absorbance was measured at 340 nm using the Absorbance Microplate Reader (SpectraMax 340PC384, USA) and the concentration was expressed in nmoles GPx/min/mg protein. (d) Effects of NaF on lipid peroxidation. The MDA in the whole homogenate was measured using the OxiSelect TBARS assay kit. The developed color was read at 532 nm using the Absorbance Microplate Reader. TBARS formation was expressed as μM TBARS 106 cells−1.
