Validation of Novel Reference Genes for Reverse Transcription Quantitative Real-Time PCR in Drought-Stressed Sugarcane
Figure 2
Pairwise variation analysis for six potential reference genes of sugarcane (α-tubulin, glyceraldehyde 3 phosphate dehydrogenase, histone H1, S-adenosylmethionine decarboxylase, ubiquitin, and 25S rRNA) based on the GeNorm analysis [17]. The addition to the two most stable genes (αTUB and GAPDH) of a random third gene (), a fourth gene (), or even more ( and ) still exhibited desirable values (basically below than 0.15).