Research Article
Validation of Novel Reference Genes for Reverse Transcription Quantitative Real-Time PCR in Drought-Stressed Sugarcane
Table 4
Relative expression rates of target genes (PFP1, AS, PIP1-1, and ACC oxidase) based on RT-qPCR with roots, cDNAs of sugarcane accessions under abiotic stress, and respective unitag regulation by SuperSAGE analysis covering droughta stress (24 h of continuous dehydration) or saltb stress (100 mM NaCl).
| ||||||||||||||||||||||||||||||||||||||||||||||||||||
AS: glutamine-dependent asparagine synthetase (EC 6.3.5.4); PFP1: pyrophosphate fructose-6-phosphate 1-phosphotransferase alpha subunit (EC 2.7.1.90); ACC oxidase: 1-aminocyclopropane-1-carboxylate oxidase (EC 1.14.17.4); PIP1-1: plasma membrane intrinsic protein. #Bulk with four accessions by each library; FC: fold change [ratio of the frequencies (normalized to 1,000,000) observed in the stressed library in relation to the control library]; &relative expression level by REST software (v.2.0.13) after the ΔΔCq method, * [27]; UR: upregulated; ns: not significant at . The time in the parentesis represents the salt stress exposition (min). |